Euphorbia hirta has been widely used by Tribal as traditional medicine in a treatment against infectious pathogens. E. hirtaLinn. is a perennial. diffusion method was used to determine the antibacterial activity on gram negative: Euphorbia hirta plant displayed an excellent antibacterial activity against K. The antibacterial and antifungal effect of Euphorbia hirta was evaluated against a panel of bacteria (Escherichia coli, Psueudomonas aeruginosa, Klebsiella.
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TA determination TA is the volume at which the test extract can be diluted with the ability to kill the microorganisms. Flavonoids of the seeds of Crotolaria retusa and Ctotolaria striata. In the current investigation E.
The isolated compounds were crystallized, weighed, and subjected to melting point m. Ethyl ether free flavonoid and ethyl acetate fractions bound flavonoids thus obtained were dried in vaccuo and weighed. Most of the extracts showed high values of TA against P. The filtrate was extracted in ethyl acetate and washed with distilled water to neutrality. Free flavonoids of stem showed bioactivity against P.
Extract discs were then placed on the seeded agar plates. Such studies could only indicate their antimicrobial potential, but are not helpful in establishing them as an antibiotic. Antimicrobial activity of selected Peruvian medicinal plants. Out of fungi; A. Infectious diseases are the leading cause of death worldwide.
The medicinal plants of Mauritius. Table 1 Rf values of spots of flavonoid extract obtained in TLC solvent system benzene: Full TA calculated against E. J Med Plant Res.
In all test microbes most susceptible organism in the investigation was C. TA and quantity of extracts from plant parts was calculated and recorded [ Table 6 ].
Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta
Rf values of spots of flavonoid extract obtained in TLC solvent system benzene: Perspectives on New Crops and New Uses. Antibacterial activity of selected Seaweads from Kovalan south West coast of India. Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Footnotes Source of Support: Gram positive bacteria S. The turbidity of the wells in the microtiter plate was interpreted as visible growth of microorganisms.
Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities. These plates were developed in an organic solvent mixture of benzene, acetic acid, and water Results of the present study reveals that all the eight tested plant extracts inhibit the growth of selected bacteria and fungi; indicating broad spectrum bioactive nature of selected plant.
Table 6 Quantity and total activity of free and bound flavonoids of Euphorbia hirta. The potential for developing antimicrobials from higher plants appears rewarding as it will lead to the development euphofbia phytomedicine to act against microbes.
This article has been cited by other articles in PMC. An international multicenter study of antimicrobial hirat and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Plant-based antimicrobials have enormous therapeutic potential as they can serve the purpose of synthetic antimicrobials. The MIC values were taken as the lowest concentration of the extracts in the well of the microtiter plate that showed no turbidity after incubation.
Antibacterial and antifungal activity was expressed in terms of activity index AI. BSAC standardized disc susceptibility testing method. Antibacillus activity of some plants used in traditional medicine of Iran. Wong E, Francis CM. Each filtrate was reextracted successively with petroleum ether fraction Iethyl ether fraction IIand ethyl acetate fraction III using separating funnel. Selected extract bound yirta of stem which showed activity against all microorganisms tested was dissolved in ethyl acetate and applied on silica gel coated 0.
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Excellent activity was shown by free and bound flavonoids of roots of E. There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action for new and reemerging infectious diseases.
Results of the present investigation indicate that E. Nil Conflict of Interest: Subramanian SS, Nagarjan S. The normal flavonoids pattern of the geraldton variety. Antibacterial activity of flavonoids of Euphorbia hirta by disc diffusion assay.
Out of the four bacteria tested, S. Int J Appl Sci Eng.